Characterization of a novel South American population of the astaxanthin producing yeast <Emphasis Type="Italic">Xanthophyllomyces dendrorhous</Emphasis> (<Emphasis Type="Italic">Phaffia rhodozyma</Emphasis>) |
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Authors: | Diego Libkind Martín Moliné Virginia de García Sonia Fontenla María van Broock |
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Institution: | (1) Laboratorio de Microbiología Aplicada y Biotecnología, Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), Universidad Nacional del Comahue, Centro Regional Universitario Bariloche (CRUB), CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Quintral 1250, 8400 Bariloche, Río Negro, Argentina |
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Abstract: | A novel population of the biotechnologically important yeast Xanthophyllomyces dendrorhous, the sexual stage of Phaffia rhodozyma, has been recently isolated for the first time in the southern Hemisphere (Patagonia, Argentina). The aim of the present
work was to phenotypically and genotypically characterize two representative strains of this new population, and assess such
strains as a potential biotechnological source of astaxanthin, fatty acids and extracellular enzymes. Minor variations were
found in physiological tests. PCR fingerprinting studies (MSP-PCR) showed the main differences between X. dendrorhous Patagonian and Type strains. Patagonian strains accumulated a xanthophyll-like pigment, which was identified as astaxanthin.
These strains showed low fatty acids content (mainly polyunsaturated fatty acids) and, of a total of six extracellular enzymes
tested, only produced amylase. Genetic differences between Patagonian and collection X. dendrorhous strains could be explained by geographic isolation and habitat specificity. |
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Keywords: | Astaxanthin Phaffia rhodozyma Xanthophyllomyces dendrorhous Patagonia Strain characterization |
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