A study of the coenzyme-binding characteristics of rabbit muscle l-glycerol 3-phosphate dehydrogenase |
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| Authors: | Philip Bentley and F Mark Dickinson |
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| Institution: | Department of Biochemistry, University of Hull, Hull HU6 7RX, U.K. |
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| Abstract: | 1. The binding of NAD(+) and NADH to glycerol 3-phosphate dehydrogenase was studied in the pH range 6.0-9.0 at 25 degrees C and in the temperature range 16-43 degrees C at pH7.0. 2. The second-order velocity constants for the combination of NADH with the enzyme in the pH range 6.0-9.0 and for the combination of NAD(+) with the enzyme at pH6.0 were determined. 3. The velocity constant for the dissociation of the enzyme-NAD(+) complex at pH6.0 was measured. |
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