Studies on the mobilization of a maize transposable family,Ac/Ds, in pepper using In Vivo transient assay system |
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Authors: | Chul Min Kim Byoung Il Je Ja Choon Koo Hai Long Piao Soon Ju Park Joo Mi Jeon Min Kyoung Kim Sung Han Park Jin Young Park Eun Jin Lee Woo Sik Chung Kon Ho Lee Kyu Young Kang Sung-Ho Lee Chang-deok Han |
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Affiliation: | (1) Division of Applied Life Science (BK21 program), Korea;(2) Plant Molecular Biology & Biotechnology Research Center (PMBBRC), Korea;(3) Environmental Biotechnology Research Center, Gyeongsang National University, 660-701 Jinju, Korea |
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Abstract: | A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and compared; one- and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts. Our data showed that both Ac and Ds elements were highly mobile in pepper cells. This is the first report suggesting that Ac/Ds mediated gene tagging system could be successfully operated in pepper. |
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Keywords: | Ac/Ds pepper transient assay |
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