Enhancement of penicillin acylase activity by cultivating immobilized Kluyvera citrophila |
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Authors: | Yasushi Morikawa Isao Karube Shuichi Suzuki |
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Institution: | (1) Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, 227 Yokohama, Japan;(2) Present address: Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., 3-6-6 Asahimachi, Machida City, Tokyo, Japan |
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Abstract: | Summary Whole cells of Kluyvera citrophila were immobilized in polyacrylamide gel. The penicillin acylase activity of immobilized whole cells was 60%–70% of native cells. When the immobilized cells were continuously cultivated for 40 h in an aerated fermentor containing peptone medium and were treated with alkali in order to remove -lactamase activity, the immobilized cells produced ampicillin up to 4.4 times faster than noncultivated cells.Ampicillin production was investigated in a column system using these cultivated immobilized whole cells. The cultivated immobilized cells showed excellent performance in continuous ampicillin production. |
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