16S rRNA gene based analysis of <Emphasis Type="Italic">Enterobacter sakazakii</Emphasis> strains from different sources and development of a PCR assay for identification |
| |
Authors: | Angelika?Lehner Taurai?Tasara Email author" target="_blank">Roger?StephanEmail author |
| |
Affiliation: | (1) Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057, Zurich, Switzerland |
| |
Abstract: | Background
E. sakazakii is considered to be an opportunistic pathogen, implicated in food borne diseases causing meningitis or enteritis especially
in neonates and infants. Cultural standard identification procedures for E. sakazakii include the observation of yellow pigmentation of colonies and a positive glucosidase activity. Up to now, only one PCR system based on a single available 16S rRNA gene sequence has been published
for E. sakazakii identification. However, in our hands a preliminary evaluation of this system to a number of target and non-target strains
showed significant specificity problems of this system. In this study full-length 16S rRNA genes of thirteen E. sakazakii strains from food, environment and human origin as well as the type strain ATCC 51329 were sequenced. Based on this sequence
data a new specific PCR system for E. sakazakii was developed and evaluated. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|