植物源物质诱导的斜纹夜蛾细胞凋亡

为了研究植物源物质对斜纹夜蛾Spodoptera litura离体培养细胞系SL-1的凋亡诱导作用，采用倒置相差显微镜观察了印楝素、喜树碱等9种物质各自对SL-1凋亡小体的浓度效应及时序性。结果表明：印楝素0.1～5.0 μg/mL和喜树碱0.5～20.0 μmol/L处理SL-1，24～48 h后均产生大量典型的凋亡小体；茶皂素、蓖麻碱、黄樟油、丹皮酚、烟碱、苦参碱和博落回碱0.1～20.0 μg/mL处理SL-1后，整个观察期72 h内均无明显凋亡小体出现，凋亡诱导作用不明显。印楝素0.75 μg/mL诱导SL-1 细胞凋亡，从凋亡小体判断，处理后0～36 h属细胞凋亡早期，36～60 h属细胞凋亡中期，60 h后为细胞凋亡晚期。喜树碱 5.0 μmol/L诱导SL-1细胞凋亡，处理后0～24 h属细胞凋亡前期，24～54 h属细胞凋亡中期，54 h后进入细胞凋亡晚期。初步认为印楝素和喜树碱对SL-1有凋亡诱导作用，并具有一定的浓度依赖性和时序性。

Induction of apoptosis by botanical components in Spodoptera litura cultured cell line

In order to determine the apoptotic inductivity of botani cal components toSpodoptera litura cultured cell line SL-1, th e concentration and time dependence of apoptotic-induce ability of nine botanic a l components, including azadirachtin and comptothecin, to SL-1 was investigated by inverted phase contrast microscope. The results showed that a great deal of a poptotic bodies appeared, one of the typical morphological characteristics of ap optosis, 24-48 h after treatments with azadirachtin at a concentration of 0.1-5.0 μg/mL and comptothecin at 0.5-20.0 μmol/L; the same changes, however, di d not appear in SL-1 cells within the whole observation period of 72 h after tre atments with tea saponin, ricinine, sassafras, paeonolum, nicotine, matrine and macleay cordata at concentrations of 0.5-20 μg/mL. Treated by azadirachtin at a concentration of 0.75 μg/mL, the early, mid and late apoptotic stages apoptosis of SL-1 cells appeared during0-36 h, 36-60 h and 60 h af ter treatment, respectively; and treated by comptochetin at a concentration of 5.0 μmol/L, these appeared during 0-24 h, 24-52 h and 54 h after treament, re spectively. It is tentatively concluded that azadirachtin and camptothecin both possess the concentration\|time-dependence induction of apoptosis to SL-1 cel ls.