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| 固定化克鲁维酵母Y-179外切菊粉酶的研究 | |
| 引用本文： | 陈玮,孙健,杜宇光,李曙光,潘建义,袁中一,赵辅昆.固定化克鲁维酵母Y-179外切菊粉酶的研究[J].工业微生物,2010,40(3):13-18. |
| 作者姓名： | 陈玮 孙健 杜宇光 李曙光 潘建义 袁中一 赵辅昆 |
| 作者单位： | 1. 浙江理工大学生命科学学院,杭州,310018 2. 中国科学院大连化学物理研究所,大连,116023 3. 中国科学院上海生命科学院生物化学与细胞生物学研究所,上海,200031 4. 浙江理工大学生命科学学院,杭州310018;中国科学院上海生命科学院生物化学与细胞生物学研究所,上海200031 |
| 摘 要： | 鹰嘴豆孢克鲁维酵母(Kluveromyces cicerisporus Y-179)分泌的糖基化菊粉外切酶经高碘酸钠氧化其分子表面的糖链产生醛基,再共价结合于氨基型固定化载体ZH-HA上,固定化酶活力达到4 000 U/g湿载体。所制备的固定化酶在pH 3.5和70℃温度下表现出最大反应活性,该固定化酶pH稳定性和热稳定性较游离酶明显提高。固定化酶在分批式反应器中重复水解菊粉50批次,活力没有明显损失,表现出良好的工作稳定性。 |
| 关 键 词： | 菊粉 菊粉酶 氧化 固定化 ZH—HA |
Studies on immobilization of exoinulinase from Kluyveromyces cicerisporus Y-179 |
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| CHEN Wei,SUN Jian,DU Yu-guang,LI Shu-guang,PAN Jian-yi,YUAN Zhong-yi,ZHAO Fu-kun.Studies on immobilization of exoinulinase from Kluyveromyces cicerisporus Y-179[J].Industrial Microbiology,2010,40(3):13-18. | |
| Authors: | CHEN Wei SUN Jian DU Yu-guang LI Shu-guang PAN Jian-yi YUAN Zhong-yi ZHAO Fu-kun |
| Institution: | 1. College of Life Science,Zhejiang Science and Technology University, Hangzhou 310018, China; 2.Dalian Institute of Chermical Physics, the Chinese Academy of Sciences, Dalian 116023, China; 3. Institute of Bioehenaistry and Cell Biology, Shanghai Institute for Life Sciences, the Chinese Academy of .Sciences, Shanghai 200031, China) |
| Abstract: | The carbohydrate chains of the glycosylated exoinulinase from Kluyoeromyces cicerisporus Y-179 was oxidized to aldehyde under mild condition by sodium periodate. Then the aldehyde attached to an amino-epoxy support ZH-HA with covalent bond. The activity of immobilized inulinase was up to 4 000 U/g wet support and showed max activity at pH 3.5 and 70℃. The immobilized enzymes also possessed an improved pH and thermal stability. After hydrolyzing inulin 50 times in a batch reactor, the immobilized inulinase showed a good operational stability. |
| Keywords: | ZH-HA |
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