A high-throughput plant DNA extraction method for marker analysis |
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Authors: | Karakousis Angelo Langridge Peter |
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Institution: | (1) Department of Plant Science, Adelaide University, 5064 Glen Osmond, South Australia |
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Abstract: | The use of molecular markers to improve crops depends on the availability of rapid and efficient DNA extraction methods. Here
we describe a simple and inexpensive method to isolate plant DNA suitable for RFLP, AFLP, and simple sequence repeat (SSR)
analysis. This procedure uses stainless steel ball bearings to grind 16 samples simultaneously using a high-speed flask shaker.
The method used in routine laboratory exercises yields 120–144 DNA extractions in a day by a single person at a cost of $0.60
(AUD) per sample, doubling the throughput of conventional methods. |
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Keywords: | AFLP ball bearing high throughput plant DNA extraction RFLP SSR |
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