Novel prolactin related mRNAs in rat pituitary cells |
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| Authors: | E J Paulssen P Alestr?m B T Johansen M Kriz L Philipson K M Gautvik |
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| Institution: | 1. Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, Debrecen, Hungary;2. Endocrine, Polypeptide and Cancer Institute, Veterans Affairs Medical Center, Miami, FL, United States;3. Institute of Pediatrics, Clinical Center, University of Debrecen, Debrecen, Hungary;4. Department of Pathology, Divisions of Hematology–Oncology and Endocrinology, Miller School of Medicine, University of Miami, Miami, FL, United States;5. Department of Medicine, Divisions of Hematology–Oncology and Endocrinology, Miller School of Medicine, University of Miami, Miami, FL, United States;6. Sylvester Comprehensive Cancer Center, University of Miami, Miami, FL, United States |
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| Abstract: | From cytoplasm of rat pituitary GH4C1 tumour cells, anti prolactin anti-serum precipitates a polypeptide with apparent molecular weight of 75.000 in addition to prolactin. In vitro translation of size fractionated RNA shows that a 82.000 molecular weight PRL-like polypeptide is encoded by a mRNA larger than the 1 kb prolactin mRNA. Northern blot analysis shows that a rat prolactin cDNA probe hybridize to a 3.2 kb RNA and a 1.5 kb RNA in addition to the 1 kb PRL mRNA. The 82.000 molecular weight translation product and the 3.2 kb mRNA is also detected in rat anterior pituitary cytoplasm. We conclude that at least one high molecular weight mRNA which code for a prolactin-like polypeptide, is present in normal rat anterior pituitary gland and in GH4C1 cells. |
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