| Abstract: | The reaction of the solvolysis of the phosphointermediate, formed in the course of the phosphotransferase reaction was studied using a number of non-specific nucleophilic agents. The methods of identification of the solvolysis products and determination of kinetic parameters were developed. The estimated dependence of the reaction of the reactivity of the nucleophilic agents versus their basicity allowed some conclusions about the transition state structure in the cases of enzyme's interaction with nucleophilic agents and protein substrate. The energy contribution of the specific interaction of the enzyme with histone H1 was evaluated. |
