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Molecular epidemiology ofCladosporium carrionii
Authors:M Kawasaki  H Ishizaki  M Miyaji  K Nishimura  T Matsumoto  S Hombo  D Muir
Institution:(1) Biological Control of Insects Research Laboratory, USDA/ARS, Research Park, P.O. Box 7629, 65205 Colombia, MO, USA;(2) Microbial Properties Research Center, USDA/ARS, Peoria, Illinois, USA
Abstract:The effects of cuticle from larvae ofTrichoplusia ni andHelicoverpa (=Heliothis)zea on expression of proteases and chitinases by germinating conidia ofNomuraea rileyi in submerged cultures were studied. Increasing the concentration ofT. ni orH. zea cuticle resulted in a 13- and 15-fold increase in protease activity, respectively. Endochitinase and N-acetylglucosaminidase activity on theT. ni andH. zea substrates increased as cuticular concentrations increased to 2.5%, then stabilized or decreased thereafter. The simultaneous expression of both proteases and chitinases suggests that they are controlled by a multiple-regulatory system.This article reports the results of research only. Mention of a proprietary product in this paper does not constitute a recommendation for use by US Department of Agriculture.
Keywords:Chitinases  Entomopathogenic fungi  Insect cuticle  Nomuraea rileyi  Proteases
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