一种降刺猬内源性脂蛋白(a)反义靶向连接物

通过酶联免疫法测定了刺猬体内脂蛋白（ａ）的含量，从中选出一些脂蛋白（ａ）水平较高的刺猬，研究脱唾液酸糖蛋白－多聚赖氨酸－反义载脂蛋白（ａ）ＲＮＡ连接物对刺猬内源性脂蛋白（ａ）的降低作用．结果表明，该连接物可明显降低刺猬体内脂蛋白（ａ）的水平，而脱唾液酸糖蛋白－多聚赖氨酸－正义载脂蛋白（ａ）ＲＮＡ连接物对刺猬体内脂蛋白（ａ）无降低作用，无脱唾液酸糖蛋白靶向的反义载脂蛋白（ａ）ＲＮＡ寡核苷酸对刺猬体内脂蛋白（ａ）的降低作用明显低于有脱唾液酸糖蛋白靶向的连接物．连接物几乎不影响刺猬体内的纤维蛋白溶酶原的活性，连接物对刺猬脂蛋白（ａ）的降低作用至少可持续１６ｈ．

Inhibition of Endogenous Lipoprotein(a) of Hedgehog by an Antisense Conjugate

Endogenous lipoprotein(a) of hedgehog was measured by ELISA,and a group of high level lipoprotein(a) of hedgehogs was obtained through this method. The aim of this paper is to identify the decreasing effect of Asor PL apo(a)antisense RNA. The result shows that, this conjugate has a distinguished decreasing effect to lipoprotein(a) concentration of hedgehog. But Asor PL apo(a)sense RNA has no decreasing effect, and non targeted apo(a)RNA only has a little decreasing effect contrasting to Asor targeted antisense apo(a)RNA conjugate. The conjugate has no decreasing effect to the plasminogen (PG)of hedgehog. The decreasing effect of this conjugate can last at least for sixteen hours.