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A simple and rapid method for the purification of the mitochondrial porin from mammalian tissues
Authors:Vito de Pinto  Girolamo Prezioso  Ferdinando Palmieri  
Institution:1. Clermont Université, Université d''Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France;2. INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 Clermont-Ferrand, France;3. Plateforme d''Exploration du Métabolisme, INRA, Centre Clermont-Ferrand–Theix, UMR 1019 , Nutrition Humaine, Saint-Genès-Champanelle, France;1. Department of Radiology and Medical Imaging, University of Virginia, Charlottesville, VA, USA;2. Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore;3. Department of Public Health Sciences, University of Virginia, Charlottesville, VA, USA;4. Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland, Baltimore, MD, USA;5. Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore, Singapore;6. Clinical Imaging Research Centre, Agency for Science, Technology and Research, Singapore, NUS-A*STAR, Singapore;1. Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS, Brazil;2. Campus Caçapava, Universidade Federal do Pampa, Caçapava, RS, Brazil;3. Centro Interdisciplinar de Pesquisas em Biotecnologia – CIPBIOTEC, Universidade Federal do Pampa, Campus São Gabriel, RS, Brazil;4. Institute of Biomedicine (IBIOMED), University of León, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain;1. Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA;2. Department of Small Animal Medicine & Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA
Abstract:A new, simple and rapid procedure for the purification of high amounts of mitochondrial porins from different tissues of mammalia is described. The method consists in a single step hydroxyapatite / celite chromatography of Triton X-100 solubilized mitochondrial membranes. For optimal purification several factors are critical such as the absence of salts, a low protein / detergent ratio and an exact hydroxyapatite / celite ratio of 2:1.
Keywords:Porin purification  Membrane protein  (Mammal)
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