成人牙周炎龈下套氧菌分离鉴定及药敏试验结果分析

分离并鉴定了329例成人牙周炎龈下优势厌氧菌群,并对不同病程中的菌群变迁、厌氧菌的药物敏感性进行了分析.成人牙周炎龈下标本中厌氧菌阳性检出率为97.9%,其中以牙龈紫质单胞菌检出率最高(38.5%),具核梭杆菌次之(18.9%).随着牙周病变程度的加重,牙龈紫质单胞菌、具核梭杆菌、产黑色素普氏菌、星群厌氧链球菌、厌氧消化链球菌的检出率增高(P＜0.05),小韦荣球菌的检出率下降(P＜0.01),表明前5种厌氧菌在AP发病过程中有重要作用,小韦荣球菌与之无关.替硝唑、甲硝唑和克林霉素对438株革兰氏阴性厌氧菌的MIC90分别为1～8,2～8和4～16 mg/L,对278株革兰氏阳性厌氧菌的MIC90分别为16～32,16～64和4～16 mg/L,表明替硝唑和甲硝唑体外抗革兰氏阴性厌氧菌效果优于克林霉素,抗革兰氏阳性厌氧菌作用不如克林霉素.

IDENTIFICATION OF SUBGINGIVAL ANAEROBIC BACTERIA FROM ADULT PERIODONTITIS PATIENTS AND ANALYSIS OF MEDICINE SENSITIVITY TESTS RESULTS OF THE ISOLATED STRAINS

A dominant anaerobic subgingival microflora from 329 cases of adult periodontitis patients was isolated and identified. Analysis showed that 97.9% of 329 periodontitis subgingival samples were anaerobic bacteria detectable. Porphyromonas gingivalis (38.5%) was the highest among the positive rated bacteria; Fusobacterium nucleatum (18.9%) was the second. The positive rate of P. gingivalis, F. nucleatum, Provotella melaninogenicus, Streptococcus constellatus , and Peptostreptococcus anaerobius increased (P<0.05) as the development of periodontal lesion, Veillonella parvula , however, decreased (P<0.01). The results suggested that the former 5 anaerobic bacteria might play an important role in development of adult periodontitis, but V. parvula was not related to the disease. The MIC 90 values of tinidazole, metronidazole, clindamycin against Gram negative and Gram positive anaerobic bacteria were measured. The medicine sensitivity was also tested.