Induction of multiple proteases during the early larval development of Artemia salina |
| |
Authors: | Carmen Osuna Asunción Olalla Antonio Sillero María AGünther Sillero Jesús Sebastián |
| |
Institution: | 1. Instituto de Enzimología del CSIC, Facultad de Medicina, Universidad Autónoma, Madrid 34, Spain;2. Departamento de Fisiología y Bioquímica, Facultad de Medicina, Universidad de Valladolid, Valladolid, Spain |
| |
Abstract: | Extracts from dormant and developing embryos of Artemia salina contain almost undetectable levels of protease activity. However, after hatching of the nauplii, there is a high increase in the activity of the extracts on different proteolytic substrates. The activities correspond to four different enzymes, A, B, C, and D, that have a sequential timing of induction during the early larval development. The enzymes show a different elution pattern after chromatography on DEAE-cellulose and a different sensitivity to protease inhibitors. Enzyme A is strongly inhibited by phenylmethylsulfonyl fluoride, while the other enzymes are mostly insensitive. Enzymes A, B, and C are sensitive at low concentrations of the soybean trypsin inhibitor. The temporal induction of these enzymes is an example of the expression, at the biochemical level, of the developmental program of Artemia salina. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|