Mitochondrial DNA synthesis in large and mature oocytes of Xenopus laevis |
| |
Authors: | Monique Barat Catherine Dufresne Hubert Pinon Monique Tourte Jean-Claude Mounolou |
| |
Affiliation: | Laboratoire de Biologie Générale, Bâtiment 400, Université Paris-Sud, 91405 Orsay, France |
| |
Abstract: | Deoxynucleosides are incorporated into mitochondrial DNA (mtDNA) of large oocytes; the rate of incorporation is about 2% of the mtDNA amount per 24 hr. When oocytes have been induced to mature in vitro with human chorionic gonadotropin (HCG), uptake and actual incorporation of thymidine decrease, although phosphorylation is enhanced. An examination of mtDNA replication shows that HCG treatment induces an increase in the relative synthesis of E-strands and an accumulation of D-loops. A similar effect is obtained by ethidium bromide treatment. Thus, gonadotropin appears to delay E-strand elongation and to synchronize mtDNA molecules at the begining of their replication cycle. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|