肾病综合征出血热的成龄鼠模型

ADULT MOUSE MODEL OF HEMORRHAGIC FEVER WITH RENAL SYNDROME

Intracerebral infection of hemorrhagic fever with renai syndrome(HF-RS ) virus proves highly pathogenic for newborn mice, but harmless for adult mice. We observed that cyclophosphamide ( CY )-immunosuppressed adult mice were Susceptible to acute infection leading to death.4-8 week old C57 mice or outbred Kunming strain mice inoculated in-tracerebrally with HFRS virus A-16 strain and immunosuppressed with four 50mg/kg CY doses at days -1, +1, + 2, + 4 (day O:virai infection), developed a lethal disease(94% mortality ) , and were mori-bund by 14-18 days postinfection. Specific virai antigen was regularly found in brain, lung, spleen, liver and kidney by indirect IF assay. His-topathologic study showed vascular changes including hyperemia, hemorr-hage, exudate, degeneratiem and necrosis of parenchymal tissues of brain, lung, liver and kidney. Based on these findings, We suggest that C57 mice or kunming strain mice may be used as an animal modei for inves-tigation of the disease.