Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column |
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| Authors: | B Vogelstein |
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| Affiliation: | 1. INQUISUR (UNS-CONICET), Department of Chemistry, Universidad Nacional del Sur, 1253 Alem Avenue, B8000CPB Bahía Blanca, Argentina;2. Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic |
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| Abstract: | A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors. |
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