Endocellular aminopeptidase from <Emphasis Type="Italic">Astasia longa</Emphasis> |
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Authors: | Yu A Rudenskaya V V Aseev G N Rudenskaya |
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Institution: | (1) Faculty of Biology, Moscow State University, Vorob’evy gory, building 40, Moscow, 119991, Russia;(2) Faculty of Chemistry, Moscow State University, Vorob’evy gory, Moscow, 119991, Russia |
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Abstract: | A new aminopeptidase was isolated from the biomass of the flagellate Astasia longa by precipitation with ammonium sulfate, gel filtration, and affinity chromatography on Arginine-Silochrome in 41% yield and with purification degree 490. The enzyme is irreversible inhibited by mercury chloride, EDTA, o-phenanthroline and, partially, bestatin and zinc chloride. It has an optimum pH 8.5 toward the hydrolysis of a synthetic chromogenic substrate Ala-pNA. The enzyme molecular mass is 45 kDa, isoelectric point 5.5, and temperature optimum 45°C. The enzyme most effectively hydrolyzes p-nitroanilides of alanine, arginine, and leucine; it is classified as metalloaminopeptidase. |
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Keywords: | Astasia longa aminopeptidase isolation properties proteases |
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