Molecular determinants responsible for the subcellular localization of HSV-1 UL4 protein |
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Authors: | Wei-wei Pan Jing Long Jun-ji Xing Chun-fu Zheng |
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Institution: | (1) State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 44 Xiaohongshan, Wuchang, Wuhan, 430071, China; |
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Abstract: | The function of the herpes simplex virus type 1 (HSV-1) UL4 protein is still elusive. Our objective is to investigate the
subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the
subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion
mutants fused with enhanced yellow fluorescent protein (EYFP), the nuclear export signals (NES) of UL4 were for the first
time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for
the granule-like cytoplasmic pattern of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm
through the NES in a chromosomal region maintenance 1 (CRM1)-dependent manner involving RanGTP hydrolysis. |
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