Protein synthetic patterns in teratocarcinoma stem cells and mouse embryos at early stages of development |
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| Authors: | Gail R Martin Sandra Smith Charles J Epstein |
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| Institution: | 1. Department of Anatomy and Cancer Research Institute, University of California, San Francisco, California, 94143 USA;2. Department of Pedatrics, University of California, San Francisco, California, 94143 USA;3. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143 USA |
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| Abstract: | The patterns of protein synthesis in teratocarcinoma stem cells (embryonal carcinoma cells) and in mouse embryos at various stages of preimplantation development were studied using SDS-polyacrylamide slab gel electrophoresis with autoradiography. Significant differences were observed in comparisons of embryonal carcinoma cells with isolated inner cell masses (ICMs) or with embryonic cells at earlier stages of development. However, no such differences in the overall pattern of protein synthesis were found when the embryonal carcinoma cells were compared with the embryonic ectoderm (that portion of the ICM which remains after endoderm differentiation). Both synthesize at least one prominent 55,000-dalton protein that is not detected in embryonic cells at earlier stages of development. This protein can thus be used as a biochemical marker of ectoderm formation during embryonic development. The pattern of protein synthesis common to embryonal carcinoma cells and embryonic ectoderm is not shared by other cultured cell types. |
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