甜茶组织培养研究

甜茶的茎段和实生苗培养在MS基本培养基中,研究植物激素对器官形成的影响,试验结果表明BA0.5-2.0毫克/升明显促进芽的形成和增殖;而对照(基本培养基)无形成芽。细胞分裂素对芽的起动是必需的。BA0.5-2.0毫克/升和GA_s1.0毫克/升配合使用,对茎段形成芽和增殖反而减少,但形成的苗较高和幼叶生长良好。通过继代培养,可繁殖大量小苗,它揭示出同一块外植体生长出许多小植株的可能,将无根苗转入含有IBA0.25-0.50毫克/升的1/2MS培养基中,能诱导生根,发展完整植株。试管苗移植土壤中,获得成功,幼苗生长良好。

STUDIES ON TISSUE CULTURE OF RUBUS SUAVISSIMUS

The stem explants and the seedlings of Rubus Suavissimus were grown on MS basal medium, the effects of plant hormones on organo-genesis were studied. The results showed that BA markedly stimulated the bud formation and proliferation in concentration ranging from 0.5 to 2.0 mg/l, but. there was no bud formation in control treatment (MS basal medium). Cytokinin was necessary for bud initiation. In using the combination of BA 0.5-2.0 mg/l and GA3 1.0 mg/1, the efficiency of bud formation and proliferation of the stem explants was decreased, but the shoot was taller and young leaf was growing well. Throught subculture numerous plantlets could be propagated. It suggests that the possibility of numerous plantlets could be secured from a single explant. When the shoots were transferred into 1/2 MS basal medium with 0.25-0.50mg/MBA, theyd eveloped into whole plantlets with root systems. Transplanting of test-tube plantlets in the soil was succeeded and plants were grown well.