Successful genotyping of microsatellites in the woolly mammoth |
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Authors: | Ishida Yasuko Roca Alfred L Fratpietro Stephen Greenwood Alex D |
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Institution: | Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA. |
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Abstract: | Genetic analyses using ancient DNA from Pleistocene and early Holocene fossils have largely relied on mitochondrial DNA (mtDNA) sequences. Among woolly mammoths, Mammuthus primigenius, mtDNA analyses have identified 2 distinct clades (I and II) that diverged 1-2 Ma. Here, we establish that microsatellite markers can be effective on Pleistocene samples, successfully genotyping woolly mammoth specimens at 2 loci. Although significant differentiation at the 2 microsatellite loci was not detected between 16 clade I and 4 clade II woolly mammoths, our results demonstrate that the nuclear population structure of Pleistocene species can be examined using fast-evolving nuclear microsatellite markers. |
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