Regulation of spinal interneuron differentiation by the paracrine action of glycine |
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Authors: | Côté Sebastien Drapeau Pierre |
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Affiliation: | Department of Pathology and Cell Biology and Groupe de recherche sur le système nerveux central, University of Montreal, Montreal, QC H3C 3J7, Canada. |
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Abstract: | Glycine and γ-aminobutyric acid (GABA) are depolarizing during early development but the purpose is unclear. We tested the effect of altering glycine signaling in zebrafish embryos by overexpressing the potassium-chloride co-transporter type 2 (KCC2) to reverse the chloride gradient or by blocking glycine receptors with strychnine or by selectively knocking down the embryonic glycine receptor (GlyR KD). Using a variety of markers we observed in all three cases a reduction of all types of spinal interneuron populations examined, indicating that glycine modulates their overall differentiation rather than choice of cell fate. Other cell populations (motor, sensory, and glial cells) were unaffected. As glycine appeared to act preceding neural and synaptic development, we examined the bandoneon (beo) mutant in which glycine receptors are functional but not clustered at synapses. Neural populations in beo embryos appeared normal, suggesting a paracrine action of circulating glycine in promoting interneuron differentiation. |
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Keywords: | glycine receptors KCC2 interneurons motor neurons sensory neurons |
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