| 过量皮质酮致原代培养的大鼠海马神经元死亡方式的研究 |
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| 作者姓名： | Ma Q Yang ZH Chao FH |
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| 作者单位： | 卫生学环境医学研究所, |
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| 摘 要： | 目的和方法：以体外原代培养的大鼠海马神经元为研究对象,采用原位染色的方法,对不同剂量的皮质酮（CORT）致海马神经元死亡的方式进行研究。结果：在CORT作用下,海马神经元不仅会发生快速的坏死,而且还会发生慢性的凋亡;并且,随着CORT剂量增大和作用时间延长,海马神经元坏死和凋亡的发生率会随之增高。结论：海马神经细胞坏死和凋亡的发生,可能与CORT抑制神经元能量代谢的程度和增高神经元对谷氨酸神经毒性的敏感性有关。
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| 关 键 词： | 应激 糖皮质激素 皮质酮 海马神经元 坏死 凋亡 |
| 文章编号： | 1000-6834(2001)01-0029-05 |
| 修稿时间： | 1999年8月30日 |
Death manners of primary cultured hippocampal neurons of rats in response to excess corticosterone exposure |
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| Ma Q,Yang ZH,Chao FH.Death manners of primary cultured hippocampal neurons of rats in response to excess corticosterone exposure[J].Chinese Journal of Applied Physiology,2001,17(1):29-33. |
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| Authors: | Ma Q Yang Z H Chao F H |
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| Institution: | Institute of Health and Environmental Medicine, Tianjin 300050. |
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| Abstract: | Aim and Methods:The death types of rat hippocampal neuronal cells in primary culture under various doses of corticosterone were questioned by in situ staining methods.Results:CORT triggered two cellular death pathways: necrosis and apoptosis. Moreover,high concentrations of CORT increased the necrotic and apoptotic percentages in dose dependent and time dependent manners.Conclusion:The factors that lead to hippocampal neuronal cells necrosis or apoptosis induced by CORT may be contributed by the impaired of energy metabolism and the vulnerability of neurons to the excess glutamate neurotoxicity. |
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| Keywords: | stress glucocorticoid(GC) corticosterone(CORT) hippocampal neuronal cells necrosis apoptosis |
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