DNA-binding domains of human fibronectin |
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Authors: | Gary K. McMaster Luciano Zardi |
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Affiliation: | 1. Department of Virology, Swiss Institute for Experimental Cancer Research, CH-1066 Epalinges sur Lausanne, Switzerland;2. Istituto Scientifico per lo Studio e la Cura dei Tumori di Genova, Viale Benedetto XV, 10, I-16132 Genova, Italy |
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Abstract: | Here we report the use of the technique of transferring proteins from polyacrylamide gels to nitrocellulose sheets to identify the DNA-binding domains of human plasma fibronectin. After separation of fibronectin and its tryptic digest on a sodium dodecylsulfate-polyacrylamide gel, the polypeptides were transferred to nitrocellulose sheets and the DNA-binding polypeptides were identified by incubation of the nitrocellul se sheets with 32P-labeled human DNA followed by autoradiography.The following results were obtained: a) only two fibronectin trypsin resistant peptides (50 and 60 kd) showed DNA-binding capacity; b) in competition experiments a 300 fold excess of E. coli DNA did not inhibit the human DNA-human fibronectin binding, indicating the specificity of the interaction. |
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Keywords: | To whom reprint requests should be addressed. |
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