A new assay for protoporphyrinogen oxidase — Evidence for a total deficiency in that activity in a heme-less mutant of |
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Authors: | Jean-Michel Camadro Danièle Urban-Grimal Pierre Labbe |
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Institution: | Laboratoire de Biochimie des Porphyrines, Université Paris VII, Tour 43, 2, Place Jussieu, 75251 Paris Cedex 05, France. |
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Abstract: | A new spectrophotometric assay for protoporphyrinogen oxidase activity has been developed, involving enzymatic generation of protoporphyrinogen in the incubation medium. This assay, more sensitive and reliable than those previously described, can be used to measure this activity in yeast mitochondrial membranes, rat liver mitochondria and membranes. By measuring protoporphyrinogen oxidase activity in different wild type and heme-mutant yeast strains, it was shown that 1) one heme-mutant was totally lacking this activity, 2) different factors might control its level in yeast. |
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Keywords: | to whom correspondance should be addressed |
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