Reconstitution of the Turkey erythrocyte adenylate cyclase sensitivity to l-epinephrine upon re-insertion of the Lubrol solubilized components into phospholipid vesicles |
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Authors: | Alan K Keenan Alma Gal Alexander Levitzki |
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Institution: | Department of Biological Chemistry The Hebrew University of Jerusalem Institute of Life Sciences 91904 Jerusalem, Israel |
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Abstract: | Turkey erythrocyte adenylate cyclase was activated by GppNHp and l-epinephrine to its stable, highly active form. In this form the enzyme could be solubilized by Lubrol-PX and subsequently re-inserted into phospholipid vesicles concomitantly with the removal of up to 99.3% of the Lubrol. The ability of GTP and l-epinephrine to reverse the GppNHp/epinephrine activated state was taken as a measure for the reappearance of hormone sensitivity in the reconstituted vesicles. An incomplete but significant reappearance of hormone sensitivity in the reconstituted adenylate cyclase was achieved. This hormone sensitivity was found to be stereospecific for (?)epinephrine. The 125I-cyanopindolol binding properties of the reconstituted β-receptor depend on the nature of the detergent and the phospholipids used in the reconstitution. |
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Keywords: | PMSF phenylmethyl-sulfonyl fluoride TME GppNHp guanylyl-imidodiphosphate CYP cyanopindolol HYP hydroxybenzylpindolol PE phosphatidyl-ethanolamine PS phosphatidyl-serine DMPC dimyristoyl-phosphatidyl-choline |
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