Isolation and characterization of androgen-dependent non-histone chromosomal protein from dorsolateral prostate of rats |
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Authors: | Yuhsi Matuo Nozomu Nishi Tetsuro Negi Yukio Tanaka Fumio Wada |
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Affiliation: | Department of Endocrinology, Kagawa Medical School, 1750, Miki-cho, Kita-gun, Kagawa 761-07, Japan |
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Abstract: | Rat prostate contains a unique androgen-dependent non-histone protein (Matuo et al. (1)). The non-histone protein was isolated in homogeneous form by extraction of nuclei from the dorsolateral prostate with 0.35 M NaCl in the presence of 1 mM PMSF and chromatography on a CM-Sepharose column. The final fraction was greater than 98% pure as judged by electrophoreses in SDS- and acid/urea-gels. The purified protein had a molecular weight of approximately 20,000, and an isoelectric point of approximately 11.5. Its absorption peak was at 276 nm and A(1%, 276nm)=9.3. The protein is characterized by the absence of cysteine, histidine and tryptophan, and by the high content of methionine, tyrosine and phenylalanine. |
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Keywords: | SDS sodium dodecyl sulfate PMSF phenylmethylsulfonyl fluoride DTT dithiothreitol |
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