Distribution of gangliosides in parenchymal and non-parenchymal cells of rat liver |
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Authors: | Kunihiro Ueno Yasunori Kushi Chiaki Rokukawa Shizuo Handa |
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Affiliation: | Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, 5-45, Yushima 1 Chome, Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | Parenchymal and non-parenchymal cells were isolated from rat liver with purities of more than 90%. Total and ganglioside sialic acid contents were higher in non-parenchymal cells than in parenchymal cells. Thin-layer chromatography of gangliosides showed that the main component in rat liver was ganglioside GM3 and that this was abundant in non-parenchymal cells. Parenchymal cells had ganglioside GD1b as the main component and less GM3 than non-parenchymal cells. These results suggested that the main ganglioside of rat liver, GM3, arises mainly from non-parenchymal cells. |
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Keywords: | TLC thin-layer chromatography HEPES N-(2-Hydroxyethyl)piperazine-N′-2-ethanesulfonic Acid |
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