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Radioimmuno-thin-layer chromatographic detection of Forssman antigen in human carcinoma cell lines |
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| Authors: | Etsuko Mori Tsuneatsu Mori Yutaka Sanai Yoshitaka Nagai |
| Affiliation: | 1. Department of Pathobiochemical Cell Research, Institute of Medical Science, University of Tokyo Shirokanedai, Minato-ku, Tokyo 108, Japan;1. Department of Immunology, Institute of Medical Science, University of Tokyo Shirokanedai, Minato-ku, Tokyo 108, Japan |
| Abstract: | Glycolipid antigen was examined by radioimmuno-thin-layer chromatography (RITLC), which is a combination of a thin-layer chromatography and radioimmunoassay. In this way Forssman antigen was studied in seven carcinoma cell lines. The usual Forssman antigen with a ceramide pentasaccharide structure was detected in cell lines of a gastric cancer and a breast cancer. In addition another glycolipid with slower mobility on thin-layer chromatography and with Forssman reactivity was found in cell lines of three gastric cancers and one lung cancer. |
| Keywords: | F Forssman TLC thin-layer chromatography C chloroform M methanol W water RITLC radioimmuno-thin-layer chromatography PBS phosphate buffered saline Abbreviations for glycosphingolipids are according to the nomenclature system of the IUPAC-IUB Commission of Biochemical Nomenclature (20) |
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