Development and characterization of monoclonal antibodies to human type III procollagen |
| |
Authors: | Nirmala SundarRaj Judith Martin Nancy Hrinya |
| |
Institution: | 1. Department of Ophthalmology, University of Pittsburgh School of Medicine, 3515 Fifth Avenue, Third Floor, Pittsburgh, Pa. 15213 USA;2. Department of Anatomy and Cell Biology, University of Pittsburgh School of Medicine, 3515 Fifth Avenue, Third Floor, Pittsburgh, Pa. 15213 USA |
| |
Abstract: | Hybridomas which secrete monoclonal antibodies against human type III procollagen have been developed. By an enzyme-linked immunosorbent assay, three of the monoclonal antibodies have been determined to be against non-helical extensions of the molecules while two of the antibodies are against helical portion of the molecules which is sensitive to bacterial collagenase action. These findings have been further confirmed by carrying out immuno-reaction of the pro α-chains transferred on nitrocellulose paper from sodium dodecyl sulfate polyacrylamide gels. These monoclonal antibodies have been found to be suitable reagents for immunohistochemical studies as well as for immunoassays of type III procollagen and collagen. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|