Selenium binding proteins in rat tissues |
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Authors: | Chandra K. Banerjee Brahma P. Sani |
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Affiliation: | Kettering Meyer Laboratory, Southern Research Institute P. O. Box 3307-A, Birmingham, Alabama 35255 USA |
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Abstract: | The 100,000 × g extracts of rat intestine and colon were incubated with Na2[75Se]O3. Chromatography of this material on a Sephadex G-100 column produced three radioactive peaks corresponding to molecular weights of 17,000, 68,000 and > 90,000. The 17,000 peak corresponded to a protein which sedimented in the 2S region of a 5–20% () linear sucrose density gradient. Selenium binding to this protein was specific, stable and sensitive to thiol inhibitors such as p-chloromercuriphenylsulfonic acid (1 mM) and iodoacetamide (2 mM). Chromatography of rat serum - [75Se] complex on Sephadex G-100 yielded only two radioactive peaks that corresponded to molecular weights of 68,000 and > 90,000. The 2S selenium binding protein of intestine and colon may mediate the biological functions of selenium in those tissues. |
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Keywords: | TMB-8 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxybenzoate |
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