Identification of several species of phospholipase inhibitory protein(s) by radioimmunoassay for lipomodulin |
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| Authors: | F. Hirata Y. Notsu M. Iwata L. Parente M. DiRosa R.J. Flower |
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| Affiliation: | 1. Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Maryland 20205 USA;2. Division of Immunology, The Johns Hopkins University, School of Medicine, The Good Samaritan Hospital, Baltimore, Maryland 21239 USA;3. Universita Degli Studi di Napoli, Instituto di Farmacologia Sperimentale, Via Leopoldo Rodino 22, 80738 Napoli, Italy;4. Wellcome Research Laboratories, Langley Court Berkenham, Kent BR 3 3BS England. |
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| Abstract: | Radioimmunoassay of lipomodulin has been developed using a monoclonal anti-lipomodulin antibody and 125I-labelled lipomodulin. Lipomodulin activity was measured in peritoneal lavage fluids obtained from rats injected with dexamethasone by radioimmunoassay and by enzymatic assay with phospholipase A2. Three species of immunoreactive substances with Mr= 40,000, 30,000 and 16,000 were found. While two species of Mr= 40,000 and 30,000 had phospholipase inhibitory activities, the species of Mr= 16,000 could inhibit phospholipase A2 only after dephosphorylation by alkaline phosphatase treatment. |
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| Keywords: | To whom all correspondence should be addressed. |
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