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| 高含氮稻田深层土壤的氨氧化古菌和厌氧氨氧化菌共存及对氮循环影响 | |
| 引用本文： | 祝贵兵.高含氮稻田深层土壤的氨氧化古菌和厌氧氨氧化菌共存及对氮循环影响[J].生态学报,2011,31(6):1487-1493. |
| 作者姓名： | 祝贵兵 |
| 作者单位： | 1. 中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085;中国科学院研究生院,北京,100049 2. 中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085 3. 中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085;北京科技大学,北京,100083 4. 中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085;兰州文通大学,兰州,730070 |
| 基金项目： | 国家自然科学基金项目(20877086); 国家973项目(2009CB421103); 国家科技重大专项(2008ZX07421-001) |
| 摘 要： | 随着海洋生态系统中的厌氧氨氧化反应和氨氧化古菌的发现,自然生态系统的氮循环过程被重新认识,但是目前尚无在陆地深层的相关报道。结合同位素示踪与分子生物学技术探索了稻田深层土壤中anammox与AOA的存在及特性。结果表明,在沼渣处理废水浇灌的高含氮稻田深层土壤中,anammox与AOA共存。通过构建克隆文库发现,此土壤中厌氧氨氧化菌的生物多样性相对较低,35个克隆序列只分为4个独立操作单元(OTU),代表序列与Genebank数据库中已探明的厌氧氨氧化菌Candidatus 'Kuenenia stuttgartiensis’的同源性超过95%;对氨氧化古菌的分析发现,20个克隆子共得到5个OTU,其与基因库中土壤/沉积物进化分支关系最近,序列的同源性部分超过98%。同位素示踪的初步结果表明,anammox产生的氮气占此土壤总氮气生成量的24.1%-29.8%。AOA与anammox的共存为anammox反应的广泛存在与发生提供了新思路。 |
| 关 键 词： | 稻田湿地 厌氧氨氧化 氨氧化古菌 深层土壤 氮循环 |
| 收稿时间： | 6/1/2010 12:00:00 AM |
| 修稿时间： | 1/11/2011 9:44:52 PM |
Coexistence, biodiversity and roles of ammonia-oxidizing archaea and anaerobic ammonium-oxidizing bacteria in deep soil layer of high nitrogen loaded paddy field |
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| Zhu Guibing.Coexistence, biodiversity and roles of ammonia-oxidizing archaea and anaerobic ammonium-oxidizing bacteria in deep soil layer of high nitrogen loaded paddy field[J].Acta Ecologica Sinica,2011,31(6):1487-1493. | |
| Authors: | Zhu Guibing |
| Institution: | State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;University of Science and Technology Beijing, Beijing 100083, China;State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;Lanzhou Jiaotong University, Lanzhou 730070, China;State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;University of Science and Technology Beijing, Beijing 100083, China;State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
| Abstract: | Anaerobic ammonium oxidation(anammox) bacteria and ammonia oxidation archaea(AOA) play a very important role in nitrogen cycle in soil as well as in the marine ecosystems.These excellent discoveries have renewed the vision on nitrogen cycle.Anammox uses nitrite as an electron acceptor to convert ammonium into dinitrogen gas.The nitrite is delivered by AOA,since they convert ammonia into nitrite.To further explore this view,the coexistence of anammox bacteria and AOA were also studied in deep soil and paddyf... |
| Keywords: | paddy field wetland anaerobic ammonium oxidation ammonia oxidation archaea deep soil layer nitrogen cycle |
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