The lipopolysaccharide of the phototrophic bacterium Ectothiorhodospira vacuolata |
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| Authors: | J Meißner D Borowiak U Fischer J Weckesse |
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| Institution: | (1) Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Schänzlestrasse 1, D-7800 Freiburg i. Br., Federal Republic of Germany;(2) Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800 Freiburg i Br., Federal Republic of Germany;(3) Fachbereich Biologie, AG Geomikrobiologie, Universität Oldenburg, Postfach 2503, D-2900 Oldenburg, Federal Republic of Germany |
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| Abstract: | The lipopolysaccharide of Ectothiorhodospira vacuolata was obtained by the phenol-water procedure. It contained a 3-O-methyl-hexose, glucose, galacturonic and glucuronic acids. The finding of d-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate (tentatively identified) suggested a core-structure. The lipid fraction of the lipopolysaccharide contained phosphate and both, 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine. The major fatty acids were amine-bound 3-OH-10:0 and 3-OH-12:0 and esterbound 14:0 and 16:0 Sodium deoxycholate gel-electrophoresis, showing a single band only, indicated R-type character of the lipopolysaccharide of Ectothiorhodospira vacuolata.Abbreviations DOC
sodium deoxycholate
- GC/MS
combined gasliquid chromatography
- PAGE
polyacrylamide gel-electrophoresis |
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| Keywords: | Ectothiorhodospira vacuotata Lipopolysaccharide Lipid A DOC-PAGE Phototrophic bacteria |
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