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A potential antimicrobial treatment against ESBL-producing <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis> using the tellurium compound AS101
Authors:Miriam Daniel-Hoffmann  Michael Albeck  Benjamin Sredni  Yeshayahu Nitzan
Institution:(1) The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 52900 Ramat-Gan, Israel;(2) The Mina and Everard Goodman Faculty of Life Sciences, The Safdié Institute for AIDS and Immunology Research, Bar-Ilan University, 52900 Ramat-Gan, Israel;(3) Department of Chemistry, Bar-Ilan University, 52900 Ramat Gan, Israel
Abstract:Due to the extensive spread of antibiotic-resistant Klebsiella pneumoniae, the non-toxic immunomodulator, ammonium trichloro (dioxoethylene-o, o′) tellurate (AS101), was introduced for the first time in this study. Eleven strains of K. pneumoniae were tested: five were extended spectrum beta lactamase (ESBL)-producing strains and six were non-ESBL-producing strains. The MIC and MBC of ten strains were 9 μg/ml AS101 and 18 μg/ml for one strain. AS101 treatment inhibited bacterial growth in a dose-dependent manner on protein-rich media. No inhibition by AS101 was observed on poorer media. In combination with β-mercaptoethanol (2-ME) or cysteamine, AS101 inhibited bacterial growth in both types of media. Growth inhibition was also shown following AS101 treatment at both lag and log phases. Our data indicate that AS101 enters the bacterium through its porins, causing bacterial destruction. The mechanism of cell death was characterized using several techniques: (a) scanning electron microscopy showed that bacteria treated with AS101 or in combination with cysteamine exhibited evidence of cell-wall damage; (b) X-ray microanalysis demonstrated damage to Na/K pumps; and (c) transmission electron microscopy demonstrated cell lysis. These phenomena suggest that AS101 has antibacterial potential against K. pneumoniae infections. B. Sredni and Y. Nitzan were equal collaborators in this research.
Keywords:Klebsiella pneumoniae            ESBL  Tellurium compound  Antimicrobial agents
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