Penicillin-binding proteins in Mycobacterium smegmatis |
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Authors: | Kunihiko Yabu Toshiro Ochiai Shunko Kaneda |
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Affiliation: | Department of Biology, Hokuriku University School of Pharmacy, Kanagawa - machi, Kanazawa 920 - 11, Japan |
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Abstract: | Abstract The penicillin-binding proteins (PBPs) of Mycobacterium smegmatis were studied. Five PBPs ranging in M r from approx. 114000 to 25000 were detected in the cytoplasmic membrane. The affinities of the PBPs for selected β-lactam antibiotics were determined. Most of the antibiotics bound to PBPs 3 and 4 at low concentrations. A penicillin-susceptible mutant and a cefmetazole-resistant mutant were isolated by selection in vitro. The PBPs of these mutants were identical to those of the parent strain. The affinity of cefmetazole for the individual PBPs was identical in each mutant. |
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Keywords: | Binding affinity of β-lactam antibiotics penicillin-susceptible mutant cefmetazole-resistant mutant |
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