Streptomyces matensis laminaripentaose hydrolase is an 'inverting' beta-1,3-glucanase. |
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| Authors: | T Nishimura C Bignon J Allouch M Czjzek H Darbon T Watanabe B Henrissat |
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| Institution: | Architecture et Fontion des Macromolécules Biologiques, CNRS, Universités d'Aix-Marseille I and II, Marseille, France. |
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| Abstract: | The laminaripentaose-producing beta-1,3-glucanase of Streptomyces matensis is a member of the glycoside hydrolase family GH-64. We have constructed and purified a recombinant hexahistidine-tagged form of the enzyme for characterisation. The enzyme, which exists as a monomer in solution, hydrolyses beta-1,3-glucan by a mechanism leading to overall inversion of the anomeric configuration. This is the first determination of the mechanism prevailing in glycoside hydrolase family GH-64 and this is the first characterisation of an 'inverting' beta-1,3-glucanase. |
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