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Localization of isopenicillin N synthase in Penicillium chrysogenum PQ-96 |
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| Authors: | W. Kurzătkowski H. Palissa H. Van Liempt H. von Döhren H. Kleinkauf W. P. Wolf W. Kuryłowicz |
| Affiliation: | (1) State Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland;(2) Institute for Biochemistry and Molecular Biology, Franklinstrasse 29, W-1000 Berlin 10, Federal Republic of Germany;(3) Federal Health Office, Robert Koch-Institute, Nordufer 20, W-1000 Berlin 65, Federal Republic of Germany |
| Abstract: | Summary For immunoelectron microscopic localization of isopenicillin N synthase (IPNS), glutaraldehyde-fixed mycelium of Penicillium chrysogenum PQ-96 was dehydrated by progressive lowering of the temperature and embedded into Lowicryl K4M at –35° C. This procedure resulted in good structural preservation such that the method of on-section labelling using antibody to IPNS from Cephalosporium acremonium CO 278 with the indirect antibody-gold technique could be successfully applied. By this method IPNS was localized in vesicular compartments belonging probably to the Golgi body and in the cell wall.Offprint requests to: W. Kurz kowski |
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