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Rapid test for determination of urea hydrolysis |
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| Authors: | S M Hussain Qadri Sabiha Zubairi H P Hawley H H Mazlaghani E G Ramirez |
| Institution: | (1) University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA;(2) Veterans Administration Medical Center, Washington, D.C., USA;(3) Hospital Regional Zona Norte Petroleos Mexicanos, Cd. Madaro, Tam., Mexico;(4) Pathology Department, Oklahoma Memorial Hospital, P.O. Box 26307, 73126 Oklahoma City, OK, USA |
| Abstract: | A urease test for the rapid determination of urea hydrolysis is described in which diluted urea agar concentrate was used in small amounts with dense inoculum of the test organisms. The method was evaluated and compared with Christensen's urea agar slants by using 728 clinical isolates of gram-negative bacteria. Of the 325 strains of urease-positive Proteus-Providencia-Morganella, 282 (87%) gave positive results within 5 min with the rapid test. Urease activity of 97% of these organisms became evident within 30 min. All 287 isolates which showed no urease activity on Christensen's urea agar also remained negative by this test. |
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