睫状神经营养因子对NO引起海马神经元毒性反应的影响

本研究采用原代培养大鼠海马神经元,观察睫状神经营养因子（ｃｉｌｉａｒｙ ｎｅｕｒｏｔｒｏｐｈｉｃ ｆａｃｔｏｒ,ＣＮＴＦ）对ＮＯ引起细胞毒性反应的影响。ＮＯ供体硝普钠与Ｓ－亚硝基－乙酰青霉胺,ＮＯＳ底物Ｌ－Ａｒｇ及钙载体ｉｏｎｏｍｙｃｉｎ,均可引起海马神经元存活率下降,ＬＤＨ漏出增加;提前２４ｈ给予不同浓度ＣＮＴＦ,均能提高神经元的存活率,减少ＬＤＨ漏出,其作用呈剂量依赖性。

Ciliary neurotrophic factor prevents NO mediated cytotoxicity in cultured hippocampal neurons

The effects of ciliary neurotrophic factor on nitric oxide (NO)-induced cytotoxicitywere examined in primary cultures of rat hippocampal neurons. The neurotoxicity wasinduced by NO donors, such as sodium nitroprusside (SNP ), s-nitroso-Nacetylpenicillamine (SNAP), NOS substrate, L-arginine (L-Arg) and ionomycin (acalcium ionophore ). Dreincubation of the cultures with ciliary neurotrophic factor(CNTF) for 24 h promoted nearonal survival and decreased the LDH efflux and the levelof NO induced by ionomycin. The protective effects of CNTF against NO cytotoxicity wereconcentration-dependent. The above findings suggest that CNTF promotes the survival ofneurons in the hippocampus by preventing NO mediated neurotoxicity.