In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu |
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Authors: | Michiko Takahashi Tomoko Nozoye Nobuyuki Kitajima Naoki Fukuda Akiko Hokura Yasuko Terada Izumi Nakai Yasuhiro Ishimaru Takanori Kobayashi Hiromi Nakanishi Naoko K Nishizawa |
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Affiliation: | 1. Graduate School of Agricultural and life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan 2. Fujita Corporation, Ono 2025-1, Atugi-shi, Kanagawa, 243-0125, Japan 3. Department of Applied Chemistry, Tokyo University of Science, 1-3, Kagurazaka, Shinjuku, Tokyo, 162-8601, Japan 4. SPring-8, JASRI, Kouto 1-1-1, Sayo-cho, Sayo-gun, Hyogo, 679-5198, Japan 5. Laboratory of Plant Biotechnology, Department of Global Agricultural Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan
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Abstract: | To investigate the flow of the metal nutrients iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu) during rice seed germination, we performed microarray analysis to examine the expression of genes involved in metal transport. Many kinds of metal transporter genes were strongly expressed and their expression levels changed during rice seed germination. We found that metal transporter genes such as ZIP family has tendency to decrease in their expressions during seed germination. Furthermore, imaging of the distribution of elements (Fe, Mn, Zn, and Cu) was carried out using Synchrotron-based X-ray microfluorescence at the Super Photon ring-8 GeV (SPring-8) facility. The change in the distribution of each element in the seeds following germination was observed by in vivo monitoring. Iron, Mn, Zn, and Cu accumulated in the endosperm and embryos of rice seeds, and their distribution changed during rice seed germination. The change in the patterns of mineral localization during germination was different among the elements observed. |
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