Efficient induction of peptide-specific cytotoxic T lymphocytes by LPS-activated spleen cells |
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Authors: | Kakugawa K Udaka K Nakashima K Inaba K Oka Y Sugiyama H Tamamura H Yamagishi H |
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Institution: | Department of Biophysics, Kyoto University, Japan. |
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Abstract: | Lipopolysaccharides of gram-negative bacteria are potent activators of B cells, dendritic cells and monocytes/macrophages. We have investigated the use of LPS-activated spleen cells as antigen-presenting cells to induce CD8+ cytotoxic T lymphocytes in vivo that are reactive to MHC class I binding peptides. Compared with resting spleen cells, CTL induction was more efficient and less variable for different peptides with LPS-activated spleen cells. Cytotoxic responses were specific for the immunized peptides and contained high affinity CD8+ T cells. The removal of dendritic cells and monocytes/macrophages by Sephadex G10 column did not show profound effects on CTL induction, indicating that B-cell blasts were largely responsible. This easily accessible method should facilitate the screening of MHC class I binding peptides to determine whether or not the host's T-cell repertoire contains reactive T cells. |
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Keywords: | LPS CTL MHC class I peptide |
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