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Cytogenetic characteristics of the cell line BHK-21 (C-13) and three of its clones |
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| Authors: | M A Urmanova V Ia Tikhonov E G Balzovskaia G P Kishchenko |
| Abstract: | Cell clones (C-9, C-24, C-36) of the cell line BHK-21 (C-13) were obtained and characterized. Compared to the original line, cells of these clones are larger in size, display an increased content of nuclear DNA and have more chromosomes. The G-bands were obtained by Giemsa staining following mild trypsin treatment. Marker chromosomes were determined in the original cell line BHK-21 (C-13) and in its large-cell clones. All the clones obtained are polyploid. |
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