Partitioning of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) from plant cell suspension culture in PEG/sodium phosphate aqueous two-phase systems |
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| Authors: | Jae-Hwa?Lee Nguyen-Hoang?Loc Tae-Ho?Kwon Email author" target="_blank">Moon-Sik?YangEmail author |
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| Institution: | (1) Department of Bioscience and Biotechnology, Silla University, 617-736 Busan, Korea;(2) Faculty of Sciences, Hue University, Hue, Vietnam;(3) Faculty of Biological Sciences, Chonbuk National University, 561-756 Chonbuk, Korea;(4) Basic Science Research Institute, Chonbuk National University, 561-756 Chonbuk, Korea |
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| Abstract: | Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems
(ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene
glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium
phosphate systems on the partition coefficient,K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system,
hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% andK
hGM-CSF of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase
with a yield of 45.66% andK
hGM-CSF of 7.64 after 2 h of incubation at room temperature. |
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| Keywords: | aqueous two-phase system hGM-CSF purification plant cell culture recombinant protein |
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