Identification,enrichment, and isolation ofThiothrix spp. from environmental samples |
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| Authors: | Brigmon Robin L. Bitton Gabriel Zam Stephen G. Martin Harris W. O'Brien Bonnie |
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| Affiliation: | (1) Department of Environmental Engineering Sciences, University of Florida, Gainesville, Florida;(2) Microbiology and Cell Science, University of Florida, Gainesville, Florida;(3) Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, Florida;(4) Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina, USA;(5) Department of Physiological Sciences, University of Florida, Box J-100144 JHMHC, 32610-0144 Gainesville, Florida, USA |
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| Abstract: | We have developed a method to enrich, isolate, and identifyThiothrix spp. in environmental samples. This procedure employs low concentrations of organic compounds, the addition of reduced sulfur compounds (sulfide or thiosulfate), and preparation with spring water that containsThiothrix spp. The enrichment enhanced identification ofThiothrix spp. by promoting deposition of intracellular sulfur granules and inhibiting overgrowth by other bacteria. The relatively high calcium content of the spring water contributed to the culture procedure. With this technique,Thiothrix spp. were observed in two activated sludge systems, a municipal water storage tank, three springs, and four underground freshwater caves in the phreatic zone of the Floridan aquifer. Two differentThiothrix cultures have been isolated from a freshwater cave and a water storage tank by this procedure. It appears that media prepared with spring water known to supportThiothrix spp. can be designed to provide highly selective methods for isolation ofThiothrix spp. from a wide range of environments.Florida Agricultural Experimentation Station, Journal Series Number R-03446. |
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