Effects of osmolytes on unfolding of chicken liver fatty acid synthase |
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| Authors: | Park Y-D Wu B-N Tian W-X Zhou H-M |
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| Institution: | (1) Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing, 100084, P. R. China;(2) Graduate School, Chinese Academy of Sciences, Beijing, 100039, P.R.China;(3) Protein Science Laboratory of the Ministry of Education, School of Life Science and Engineering, Tsinghua University, Beijing, 100084, P.R.China |
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| Abstract: | Urea-induced aggregation of chicken liver fatty acid synthase acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating,oxoacyl- and enoyl-reducing and thioester-hydrolyzing), EC 2.3.1.85 ] was studied. The aggregation was facilitated at increased ionic strength. Methyl- -cyclodextrin and some osmolytes, such as glycerol, sucrose, proline, glycine, and heparin, could effectively prevent the aggregation, implying an artificial chaperone role of those substances during fatty acid synthase unfolding. The osmolytes also protected the enzyme from inactivation. |
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| Keywords: | fatty acid synthase urea denaturation aggregation osmolyte artificial chaperone |
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