Intraspecific mitochondrial DNA variation in the Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) |
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Authors: | A. M. L. Azeredo-Espin R. F. W. Schroder G. K. Roderick W. S. Sheppard |
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Affiliation: | 1. Departameto de Genética e Evolu??o e Centro de Biologia Molecular e Eng. Genética, Universidade Estadual de Campinas, C.P. 6109- 13083-970, Campinas, SP, Brasil 2. Bee Research Laboratory, ARS-USDA, Building 476, Barc-East, 20705, Beltsville, Maryland 3. Insect Biocontrol Laboratory, ARS-USDA, Building 011-A, Barc-West, 20705, Beltsville, Maryland 4. Center for Conservation Research and Training, 3050 Maile Way, Gilmore 310, University of Hawaii, 96822, Honolulu, Hawaii 5. Department of Entomology, 166 FSHN Building, Washington State University, 99164, Pulman, Washington
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Abstract: | Restriction endonuclease analyses of mitochondrial DNA (mtDNA) were used to examine genetic variability and population structure inLeptinotarsa decemlineata (Say). A group of three enzymes, EcoRI, HpaI, and PstI, was used to reveal polymorphism both within and among some of the 10 populations tested, yielding 16 haplotypes in combination. The frequencies of these 16 haplotypes differed significantly across geographic regions, indicating some partitioning of mtDNA haplotypes. Estimates of mtDNA sequence divergence (δ) between haplotypes ranged from 0.016 to 0.135%, suggesting local differentiation of mtDNA in some populations. Analysis of these data suggests that Texas was colonized by more than one mtDNA lineage, most likely originating in Mexico. We hypothesize that a larger founder size for the initial introductions or high levels of variability in the parent population at the edge of the CPB expanding range led to the initial partitioning of haplotypes observed in samples from Texas. |
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Keywords: | Leptinotarsa decemlineata Colorado potato beetle mitochondrial DNA genetic variation heteroplasmy |
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