Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate |
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Authors: | Chowdhury Sougata Roy Basak Ratan Kumar Sen Ramkrishna Adhikari Basudam |
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Affiliation: | a Materials Science Centre, Indian Institute of Technology Kharagpur, West Bengal 721302, India b Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India |
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Abstract: | Bacillus megaterium RB-05 was grown on glucose and on “tossa-daisee” (Corchorus olitorius)-derived jute, and production and composition of extracellular polysaccharide (EPS) were monitored. An EPS yield of 0.065 ± 0.013 and of 0.297 g ± 0.054 g−1 substrate after 72 h was obtained for glucose and jute, respectively. EPS production in the presence of jute paralleled bacterial cellulase activity. High performance liquid chromatography (HPLC), matrix assisted LASER desorption/ionization-time of flight (MALDI-ToF) mass spectroscopy, and fourier transform infrared (FT-IR) spectroscopy demonstrated that the EPS synthesized in jute culture (JC) differed from that synthesized in glucose mineral salts medium (GMSM). While fucose was only a minor constituent (4.9 wt.%) of EPS from GMSM, it a major component (41.9 wt.%) of EPS synthesized in JC. This study establishes jute as an effective fermentation substrate for EPS production by a cellulase-producing bacterium. |
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Keywords: | Bacillus megaterium RB-05 Extracellular polysaccharide Jute Composition |
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